Cellular
Reprogramming
Laboratory
PUBLICATIONS
1
Research Papers
Park SM, Lee JH, Ahn KS, Shim HW, Yoon JY, Hyun J, Lee JH, Jang S, Yoo KH, Jang YK, Kim TJ, Kim HK, Lee MR, Jang JH, Shim H*, Kim HW*. 2023. Cyclic stretch promotes cellular reprogramming process through cytoskeletal-nuclear mechano-coupling and epigenetic modification. Adv Sci 19:e2303395 (*corresponding authors).
Yoon JY, Mandakhbayar N, Hyun J, Yoon DS, Patel KD, Kang K, Shim H, Lee HH, Lee JH, Leong KW, Kim HW. 2022. Chemically-induced osteogenic cells for bone tissue engineering and disease modeling. Biomaterials 289:121792.
Chung SY, Carpena NT, Mun S, Jung JY, Chung PS, Shim H, Han K, Ahn JC, Lee MY. 2020. Enhanced inner ear organoid formation from mouse embryonic stem cells by photobiomodulation. Mol Ther Methods Clin Dev 27:556-567.
Kim YJ, Kwon HJ, Byun HS, Yeom D, Choi JH, Kim JH, Shim H. 2017. Surveyor assay to diagnose persistent Müllerian duct syndrome in Miniature Schnauzers. J Vet Sci 18:547-549.
Kim YJ, Ahn KS, Kim M, Kim MJ, Ahn JS, Ryu J, Heo SY, Park SM, Kang JH, Choi YJ, Shim H. 2017. Alpha-1,3-galactosyltransferase-deficient miniature pigs produced by serial cloning using neonatal skin fibroblasts with loss of heterozygosity. Asian-Australas J Anim Sci 30:439-445.
Kim YJ, Kim TH, Kim M, Kim MJ, Kim HW, Shim H. 2016. Complete reduction of p53 expression by RNA interference following heterozygous knockout in porcine fibroblasts. In Vitro Cell Dev Biol Anim 52:736-741.
Perez RA, Choi SJ, Han CM, Kim JJ, Shim H, Leong KW, Kim HW. 2016. Biomaterials control of pluripotent stem cell fate for regenerative therapy. Prog Mater Sci 82:234-293.
Ryu J, Kim M, Ahn JS, Ahn KS, Shim H. 2016. Nuclear transfer using human CD59 and IL-18BP double transgenic fetal fibroblasts. J Embryo Transfer 31:1-7.
Kim MJ, Kim YJ, Shim H. 2016. Treatment of exogenous GDF9 and BMP15 during in vitro maturation of oocytes increases the cell number of blastocysts in pigs. J Embryo Transfer 31:9-12.
Jang H, Jang WG, Kim EJ, Do M, Oh KB, Hwang S, Shim H, Choo YK, Kwon DJ, Lee JW. 2015. Methylation and expression changes in imprinted genes H19 and Igf2 during serial somatic cell nuclear transfer using piglet fibroblasts. Anim Cells Systems 19:46-53.
Park HM, Kim YW, Kim KJ, Kim YJ, Yang YH, Jin JM, Kim YH, Kim BG, Shim H,* Kim YG.* 2015. Comparative N-linked glycan analysis of wild-type and α1,3-galactosyltransferase gene knock-out pig fibroblasts using mass spectrometry approaches. Mol Cells 38:65-74 (*corresponding authors).
Kim YJ, Ahn KS, Kim M, Kim MJ, Park SM, Ryu J, Ahn JS, Heo SY, Kang JH, Choi YJ, Choi SJ, Shim H. 2014. Targeted disruption of Ataxia-telangiectasia mutated gene in miniature pigs by somatic cell nuclear transfer. Biochem Biophys Res Commun. 452:901-905.
Park JY, Park MR, Bui HT, Cho SG, Park C, Kim HM, Kang MJ, Park SB, Kang YK, Lee JW, Shim H, Lee KK, and Kim JH. 2012. Alpha-1,3 galactosytrasnsferase deficiency in germfree miniature pig increased non-gal N-glycolylneuraminic acids as the xenoantigenic determinant in pig-human xenotransplantation. Cell Reprogram 14:353-363.
Do M, Jang WG, Hwang JH, Jang H, Kim EJ, Jeong EJ, Shim H, Hwang SS, Oh KB, Byun SJ, Kim JH, Lee JW. 2012. Inheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT). Biochem Biophy Res Commun 424:765-770.
Lee BH, Ahn KS, Heo SY, Shim H. 2012. Hypomethylation of DNA in nuclear transfer embryos from porcine embryonic germ cells. J Embryo Transfer 27:113-119.
Hwang S, Oh GB, Kim DH, Woo JS, Shim H, Yun IK, Park JK, Im GS. 2012. Production of α1,3-galactosyltransferase (GalT) double knockout (-/-) transgenic pigs for xenotransplantation. Reprod Dev Biol 27:9-14.
Shin S, Ahn KS, Choi SJ, Heo SY, Shim H. 2012. In vitro development of nuclear transfer embryos derived from porcine embryonic germ cells and their descendent neural precursor cells. Zygote 20:9-15.
Kim YJ, Ahn KS, Kim M, Shim H. 2011. Comparison of potency between histone deacetylase inhibitors trichostatin A and valproic acid on enhancing in vitro development of porcine somatic cell nuclear transfer embryos. In Vitro Cell Dev Biol Anim 47:283-289.
Ahn KS, Kim YJ, Kim M, Lee BH, Heo SY, Kang MJ, Kang YK, Lee JW, Lee KK, Kim JH, Nho WG, Hwang SS, Woo JS, Park JK, Park SB, Shim H. 2011. Resurrection of an alpha-1,3-galactosyltransferase gene-targeted miniature pig by recloning using postmortem ear skin fibroblasts. Theriogenology 75:933-939.
Min SH, Lee SH, Shim H, Park JS, Lee YI, Kim HW, Hyun JK. 2011. Development of complete thoracic spinal cord transection model in rats for delayed transplantation of stem cells. Spine 36:E155-E163.
Ahn KS, Won JY, Park JK, Sorrell AM, Heo SY, Kang JH, Woo JS, Choi BH, Chang WK, Shim H. 2010. Production of human CD59-transgenic pigs by embryonic germ cell nuclear transfer. Biochem Biophy Res Commun 400:667-672.
Kim M, Ahn KS, Kim YJ, Shim H. 2009. Factors affecting primary culture of nuclear transfer blastocysts for isolation of embryonic stem cells in miniature pigs. Reprod Dev Biol 33:133-137.
Won JY, Ahn KS, Sorrell AM, Shin S, Heo SY, Kang JH, Park JK, Chang WK, Shim H. 2009. Cytolytic assessment of hyperacute rejection and production of nuclear transfer embryos using hCD46-transgenic porcine embryonic germ cells. Zygote 17:101-108.
Yoon JY, Kwon MS, Kang JH, Ahn KS, Kim NH, Kim JH, Kim T, Shim H. 2009. Production of bovine nuclear transfer embryos using fibroblasts transfected with single-chain human follicle-stimulating hormone gene. Asian Australas J Anim Sci 22:168-173.
Ahn KS, Jeon SJ, Jung JY, Kim YS, Kang JH, Shin S, Choi T, Choi SJ, Chung P, Shim H. 2008. Isolation of embryonic stem cells from enhanced green fluorescent protein-transgenic mouse and their survival in the cochlea after allotransplantation. Cytotherapy 10:759-769.
Heo SY, Ahn KS, Kang JH, Shim H. 2008. Enhanced green fluorescent protein gene under the regulation of human Oct4 promoter as a marker to identify reprogramming of human fibroblasts. Reprod Dev Biol 32:137-142.
Shim SW, Han DW, Yang JH, Lee BY, Kim SB, Shim H, Lee HT. 2008. Derivation of embryonic germ cells from post migratory primordial germ cells, and methylation analysis of their imprinted genes by bisulfite genomic sequencing. Mol Cells 25:358-367.
Kang JH, Ahn KS, Heo SY, Won JY, Shim H. 2008. Gene targeting in mouse embryos mediated by RecA and modified single-stranded oligonucleotides. In Vitro Cell Dev Biol Anim 44:57-62.
Shim SW, Kim YH, Lee HT, Shim H. 2008. Effects of sperm membrane disruption and electrical activation of oocytes on in vitro development and transgenesis of porcine embryos produced by intracytoplasmic sperm injection. Asian Australas J Anim Sci 21:358-363.
Ahn KS, Won JY, Heo SY, Kang JH, Yang HS, Shim H. 2007. Transgenesis and nuclear transfer using porcine embryonic germ cells. Cloning Stem Cells 9:461-468.
Ahn KS, Won JY, Heo SY, Kang JH, Shim H. 2007. Comparison of in vitro development of porcine embryos derived from transfer of embryonic germ cell nuclei into oocytes by electrofusion and piezo-driven microinjection. Reprod Dev Biol 31:127-131.
Won JY, Ahn KS, Heo SY, Shim H. 2007. Use of porcine embryonic germ cells for xenotransplantation research. Reprod Dev Biol 31(Suppl):7-16.
Choi BR, Koo BC, Ahn KS, Kwon M, Kim JH, Cho SK, Kim KM, Kang JH, Shim H, Lee H, Uhm SJ, Lee HT, Kim T. 2006. Tetracycline-inducible gene expression in nuclear transfer embryos derived from porcine fetal fibroblasts transformed with retrovirus vectors. Mol Reprod Dev 73:1221-1229.
Kim SS, Yoon JY, Ahn KS, Shim H. 2005. Diagnosis of freemartinism in Korean Native Cattle by amplification of two different male-specific DNA sequences. Reprod Dev Biol 29:269-271.
Kim SS, Park YS, Park YM, Park HD, Shim H, Kim NH. 2005. Effects of technical aspects in practitioner on the paregnancy and abortion after transfer of in vitro produced Korean native cow embryos. Korean J Embryo Transfer 20:9-15.
Park JS, Kim HY, Kim HW, Chae GN, Oh HT, Park JY, Shim H, Seo M, Shin EY, Kim EG, Park SC, Kwak SJ. 2005. Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells. Mech Ageing Dev 126:551-559.
Won JY, Kang JH, Shim H. 2004. Polscope-assisted enucleation for nuclear transfer in mice. Reprod Dev Biol 28:257-260.
Kwon MS, Koo BC, Choi BR, Lee HT, Kim YH, Ryu WS, Shim H, Kim JH, Kim T. 2004. Development of transgenic chickens expressing enhanced green fluorescent protein. Biochem Biophys Res Commun 320:442-448.
Shim H. 2004. Use of somatic cell nuclear transfer technology to produce human follicle-stimulating hormone (hFSH) transgenic cows. Korean J Embryo Transfer 19(Suppl):39-42.
Rui R, Shim H, Moyer AL, Anderson D, BonDurant RH, Anderson GB. 2004. Attempts to enhance production of chimeras from porcine embryonic germ cells and preimplantation embryos. Theriogenology 61:1225-1235.
Kwon MS, Koo BC, Shim H, Park CS, Lee SH, Kim T. 2003. Transfer and expression of the recombinant hFSH gene in CHO cells and transgenic chicken using retrovirus vector system. Korean J Anim Reprod 27:197-206.
Cho J, Park S, Chung H, Shim H, Lee B, Rhee K, Kang S, Han J, Lee C, Hwang W, Lim J. 2002. Improved development of ICR mouse 2-cell embryos by the addition of amino acids to a serum-, phosphate- and glucose-free medium. J Vet Med Sci 64:797-801.
Bertolini M, Beam SW, Shim H, Bertolini LR, Moyer AL, Famula TR, Anderson GB. 2002. Growth, development and gene expression by in vivo- and in vitro-produced day 7 and 16 bovine embryos. Mol Reprod Dev 63:318-328.
Shim H. 2002. Embryonic stem cells: From knockout mice to cell replacement therapy. Exp Mol Med 34(Suppl):99-110.
Shin MR, Park SW, Shim H, Kim NH. 2002. Nuclear and microtubule reorganization in nuclear-transferred bovine embryos. Mol Reprod Dev. 62:74-82.
Park SW, Shin MR, Kim YH, Shim H, Kim NH. 2001. Development of nuclear transfer embryos using somatic cell nuclei from Korean Native Cattle (Hanwoo) with high genetic value. Korean J Anim Reprod 25:51-62.
Jun SH, Shim H, Chung HM, Lee BC, Lee ES, Ko JJ, Shin T, Park C, Hwang WS, Cha KY, Lim JM. 2000. Improvement of somatic cell nuclear transfer technology for the production of disease model mouse: I. Optimization of oocyte enucleation and reconstruction. Korean J Embryo Transfer 15:247-253.
Uhm SJ, Chung HM, Kim C, Shim H, Kim NH, Lee HT, Chung KS. 2000. In vitro development of porcine enucleated oocytes reconstructed by porcine fetal fibroblasts and cumulus cells. Theriogenology 54:559-570.
Kim NH and Shim H. 2000. Intracytoplasmic sperm injection in pigs. Embryo Transfer Newsletter 18(2):10-13.
Roh S, Shim H, Hwang WS, Yoon JT. 2000. In vitro development of green fluorescent protein (GFP) transgenic bovine embryos after nuclear transfer using different cell cycles and passages of fetal fibroblasts. Reprod Fertil Dev 12:1-6.
Choi SJ, Shim H, Anderson GB. 1999. Lack of stage-specific embryonic antigen-1 expression by bovine embryos and primordial germ cells. J Dairy Sci 82:516-519.
Shim H, Anderson GB. 1998. In vitro survival and proliferation of porcine primordial germ cells. Theriogenology 49:521-528.
Shim H, Gutierrez-Adan A, Chen LR, BonDurant RH, Behboodi E, Anderson GB. 1997. Isolation of pluripotent stem cells from cultured porcine primordial germ cells. Biol Reprod 57:1089-1095.
Shim H, Kim JH, Lee BC, Kim JB, Park HY, Chung KS. 1988. Studies on the production and applications of monoclonal antibodies to H-Y. Korean J Anim Sci 30:411-418.
Shim H, Ko JJ, Kim JB, Park HY, Chung KS. 1986. Sex ratio of mouse offspring following in vitro treatment of H-Y antibody to mouse embryos prior to transfer. Korean J Anim Sci 28:759-764.
Ko JJ, Shim H, Kim JB, Park HY, Chung KS. 1986. Optimal condition and interspecific cross reaction of H-Y antibody activity. Korean J Anim Reprod 10:168-174.
Ko JJ, Shim H, Kim JB, Park HY, Chung KS, Lee KK. 1986. Effect of H-Y antibody on in vitro development of mouse embryos. Korean J Anim Reprod 10:42-48.
2
Patents
Lee BC, Ahn C, Kim GA, Shim H, Ahn KS, Lee SG, Kim SJ, Lee C, Heo SY, Choi I, Kang JT, Cho BR. 2021. BTK knockout disease model pig and uses thereof. Korean Patent No. 10-2270145, issued on June 22, 2021.
Lee JW, Lee KK, Jang MR, Kang YK, Kang MJ, Kim JH, Seong HH, Shim H, Hwang SS. 2012. Genetically-modified cell line for producing cloned miniature pigs for xenotransplantation and method for preparing the same. Korean Patent No. 10-1149475, issued on May 17, 2012.
Lee KK, Kang MJ, Lee JW, Jang MR, Kim HM, Lee SM, Kim JH, Seong HH, Shim H. 2011. Alpha 1,3-galactosyltransferase gene targeting vector and uses thereof. Korean Patent No. 10-1068479, issued on September 22, 2011.
Kang YK, Kang MJ, Joo JH, Lee SM, Park SB, Shim H, Kim JH, Lee JW, Jang MR. 2011. Methods for production of knock-in somatic cells of DAF gene in alpha 1,3-galactosyltransferase gene locus. Korean Patent No. 10-1048426, issued on July 5, 2011.
Lee KK, Chang M, Lee JW, Kang YK, Kang MJ, Kim JH, Seong HH, Shim H, Hwang S. 2008. Genetically-modified cell line for producing cloned miniature pigs for xenotransplantation and method for preparing the same. PCT Application No. PCT/KR2008/007087.
Hyun JK, Min SH, Lee, SH, Shim H, Lee YI, Kwak WB, Park JS. 2008. Method for making the same, and using the complete transection model of spinal cord injury in rodents. Korean Patent No. 10-0823935, issued on April 15, 2008.
Kim T, Shim H. 2008. Expression vector including recombinant hFSH gene. Korean Patent No. 10-0587535, issued on May 30, 2006.
Anderson GB, Shim H. 2003. Embryonic germ cells, method for making same, and using the cells to produce a chimeric porcine. United States Letters Patent No. 6,545,199 B1, issued on April 8, 2003.
Anderson GB, Shim H. 2001. Embryonic germ cells, method for making same, and using the cells to produce a chimeric porcine. United States Letters Patent No. 6,194,635 B1, issued on October 27, 2001.
Anderson G.B, Shim H. 1997. Ungulate embryonic stem-like cells, making and using the cells to produce a transgenic ungulate. Patent Cooperation Treaty Publication, World Intellectual Property Organization. WO 97/25413.
Anderson GB, Shim H. 1997. Ungulate embryonic stem-like cells, method for making and using the cells to produce transgenic ungulates. Patent Cooperation Treaty Publication, World Intellectual Property Organization. WO 97/25412.